悠洋棋牌

發布時間:2011-08-20         浏覽次數:5982

 顧穎,博士  GU Ying, Ph.D.

 副教授,碩士生導師 Associate Professor

 電話:+86-592-2187880

 傳真:+86-592-2181258

  E-mailguying@xmu.edu.cn


簡曆 (Education and Work experience):

1996年,廈門大學生物學專業,學士學位;

2006年,廈門大學生化與分子生物學專業/細胞生物學專業,博士學位;

2006年,廈門大學生命科學學院助理教授、碩士生導師;

2013年,廈門大學生命科學學院副教授;

B.S.  Biochemistry, Xiamen University, 2000

Ph.D. Biochemistry & Molecular Biology/Cell Biology, Xiamen University, 2006

Assistant Professor, School of Life Sciences, Xiamen University, 2006

Associate Professor, School of Life Sciences, Xiamen University, 2013


研究領域 (Research Area):

1、抗病毒的單抗篩選:利用細胞模型和重組抗原等進行抗病毒的單抗篩選,如HIV中和單抗、識別免疫優勢表位單抗等的篩選。

2、基于表位的抗原研究:利用基因工程重組技術表達獲得含有功能表位的抗原或顆粒化抗原。

3、功能性表位研究:應用生物傳感器、分析超離、微量熱等方法研究抗原-抗體的相互作用,嘗試解析免疫複合物的空間結構,鑒定功能性表位的特征。

4、Generation  of monoclonal antibodies against different viral targets : based on functional screening by virus-infection cell model, recombinant antigen  reactivity and et. al, focusing on HIV neutralizing monoclonal antibodes,  esp. those with broad neutralizing activity

5、Identification  and characterization of key epitopes on viral antigens: Design and      preparation of recombinant antigens harbouring functional epitopes

6、Functional  characterization of epitopes: epitope mapping, structural analysis and      function interpretation by various methods including SPR-based assay,  analytical ultracentrifugation, microcalorimetry, and structural determination of antibody-antigen complexes


代表性論文(Selected Publications)(* Corresponding author)

1、Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K,  Zhang J, Ng MH, Hew CL, Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization of  hepatitis E virus by a cross-genotype antibody. Cell Res. 2015  May;25(5):604-20.

2、Wang D, Li Z, Xiao J, Wang J, Zhang L, Liu Y, Fan F,  Xin L, Wei M, Kong Z, Yu H, Gu Y,     Zhang J, Li S, Xia N. Identification of Broad-Genotype HPV L2  Neutralization Site for Pan-HPV Vaccine Development by a  Cross-Neutralizing Antibody. PLoS One. 2015 Apr     23;10(4):e0123944.

3、Gu Y, Cao F, Wang L, Hou  W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel  Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody     A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013 Apr     19;8(4):e61314.

4、Yang C, Pan H, Wei M, Zhang X, Wang N, Gu Y, Du H, Zhang J,Li S*, Xia N*.Hepatitis Evirus capsid protein assembles in 4M urea in the presence of salts.Protein Sci. 2013  Mar;22(3):314-26.

5、Gu Y, Hou W, Xu C, Li S, Shih JW, Xia N*. The enhancement of RNAi  against HIV in vitro and in vivo using H-2K(k) protein as a sorting  method. J Virol Methods. 2012 Jun;182(1-2):9-17.

6、Shen L1, Gu Y 1, Sun L, Yang Y, Wang F, Li Y, Bi S*. Development of a hepatitis  delta virus antibody assay for study of the prevalence of HDV among  individuals infected with hepatitis B virus in China. J Med Virol. 2012  Mar;84(3):445-9. (co-1st)

7、Tang X, Yang C, Gu  Y, Song C, Zhang X, Wang Y, Zhang J, Hew CL,Li S*, Xia N*, Sivaraman  J*. Structural basis for the neutralization and genotype specificity of  hepatitis E virus. ProcNatl Acad Sci U S A. 2011 Jun 21;108(25):10266-71.

8、Yu H,Li S*, Yang C, Wei M, Song C, Zheng Z, Gu Y, Du H, Zhang J, Xia N*. Homology model and potential virus-capsid binding site of a putative HEV receptor Grp78. J Mol Model. 2011 May;17(5):987-95.

9、Zheng Z, Luo W*, Song H, Chen Y, Xiong J, Chen Y, Gu Y, Chen H, Shih JW, Zhang J, Xia N*. Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins. Arch Virol. 2010;155(1):19-26.

10、Li S, Tang X, Seetharaman J, Yang C, Gu Y, Zhang J, Du H, Shih JW, Hew CL, Sivaraman J*, Xia N*. Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction.PLoS Pathog. 2009 Aug;5(8):e1000537.

11、Xu CY, Gu Y*, Hou WH, Que YQ, Gao SG, Cheng T, Xia NS*. Tetracysteine as a Reporter for Gene Therapy. Biomed Environ Sci. 2009 Dec;22(6):496-501.

12、Li S, Zhang J, He Z, Gu Y, Liu R, Lin J, Chen Y, Ng M, Xia N*. Mutational analysis     of essential interactions involved in the assembly of hepatitis E virus capsid.J Biol Chem. 2005 Feb 4;280(5):3400-6.

13、Yang HJ, Chen M, Cheng T, He SZ, Li SW, Guan BQ, Zhu ZH, Gu Y, Zhang J, Xia NS.Expression and immunoactivity of chimeric particulate antigens of receptor binding site-core antigen of hepatitis B virus. World J Gastroenterol. 2005 Jan 28;11(4):492-7.

14、Zhang J, Gu Y,  Ge SX, Li SW, He ZQ, Huang GY, Zhuang H, Ng MH, Xia NS. Analysis of  hepatitis E virus neutralization sites using monoclonal antibodies directed against a virus capsid protein. Vaccine. 2005 Apr  22;23(22):2881-92.

15、Gu Y, Zhang J, Wang YB, Li SW, Yang HJ, Luo WX,  Xia NS*. Selection of a peptide mimicking neutralization epitope of  hepatitis E virus with phage peptide display technology. World J Gastroenterol. 2004 Jun 1;10 (11):1583-8.

16、Zhang J, Ge SX, Huang GY, Li SW, He ZQ, Wang YB, Zheng YJ, Gu Y, Ng MH, Xia NS. Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model. J Med Virol. 2003 Dec;71(4):518-26.


榮譽、獎勵及參加學術團體的情況(Honors and award):

       主持3項國家自然科學基金項目、1項國家科技重大專項子課題,1项福建省科技重大项目,并作为主要参与人参加多项国家级、省部级重大重点科研項目;获得中国高校科技进步一等奖(2001),國家技術發明二等獎(2010),中國專利金獎(2012),廈門市科學技術進步一等獎(2010),廈門大學第四屆青年教師教學技能比賽一等獎(2008),廈門大學第三屆青年教師教學技能比賽二等獎(2007。申請發明專利11項,國際發明專利8項,獲得授權4項;爲主參與研制戊肝疫苗、宮頸癌疫苗、艾滋診斷試劑等新藥。

WIPO-SIPO  Award for Chinese Outstanding Patented Invention (2012)

State  Technological Invention Award (2010)